Transitory noradrenergic and peptidergic nerves in the cat kidney

Abstract

Indirect immunohistochemical methods were used to visualize nerves immunoreactive for tyrosine hydroxylase (TH), dopamine beta hydroxylase (DBH), neuropeptide Y, (NPY) and calcitonin gene-related peptide (CGRP) in sections of the kidneys of cats of different ages. Nerve terminals immunoreactive for TH, DBH and NPY innervated interlobar veins and the renal arterial tree including medullary vascular bundles of cats of each age studied. Most nerve terminals immunoreactive for CGRP innervated interlobar arteries. In kidneys of cats 2 to 10 weeks old, TH- and DBH-immunoreactive axons formed elaborate plexuses that were distributed throughout much of the outer two thirds of the inner medulla. Inner medullary NPY-immunoreactive nerve terminals formed sparse plexuses by comparison, thus suggesting a large population of TH-immunoreactive nerve terminals not immunoreactive for NPY. Plexuses immunoreactive for CGRP also innervated the inner medullae of young cats. Some inner medullary axons appeared degenerate in 8 and 10 week old cats, and no inner medullary nerve terminal plexuses were visualized in 12 week old or adult cats. Cell death or paring of axons resulting from mechanisms intrinsic to the neuronal population or from a change in trophic factors secreted or expressed by cells in the medulla may effect the loss of inner medullary nerve terminals in the kidneys of young cats.