Abstract
Removal of cumulus cells surrounding oocytes, denudation, is a necessary step prior to some Assisted Reproductive Technology procedures. An important aspect during the chemical/physical removal of cumulus cells is to minimize the risk of fractured zona pellucida (FZ) damage. In our labratory, two denudation medias have been used to facilitate this action: Irvine Scientific's Hyaluronidase Solution (HYAL) and Origio's ICSI Cumulase® (CUMU). Over a 12 month time period we initially used HYAL, then transitioned to CUMU and finally returned back to HYAL.
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