Transition of spatio-temporal distribution of soil enzyme activity after straw incorporation: From rhizosphere to detritusphere

Abstract

The rhizosphere and detritusphere are hotspots of soil enzyme-mediated microbial processes, but little is known about their spatio-temporal distribution and interactions in situ, especially after straw incorporation. To answer this question, we planted maize in rhizoboxes with three wheat straw localizations: a) straw incorporated in the upper 2 cm of soil (straw localized); b) straw mixed with all the soil (straw homogenized); and 3) without straw (control). Zymography was used to investigate the spatio-temporal distribution of soil C- and N- acquiring enzyme activities in the rhizosphere (living roots) and detritusphere (straw induced), which depends on straw localization (localized vs. homogenized) and time (7 vs. 15 days after planting). The hotspot area of enzyme activities was similar in the detritusphere compared to the rhizosphere at the early stage (day 7), suggesting the importance of detritusphere in driving C and N cycling. Compared with homogenized straw, higher C- and N-acquiring enzyme activities in detritusphere were observed in the treatment with localized straw, which indicates that straw localization had a marked impact on the enzyme activity in the detritusphere. Although enzyme activities in rhizosphere and detritusphere decreased with time from straw application, straw incorporation increased the enzyme activities in the rhizosphere (especially with localized straw) compared to control at the late stage (day 15). Furthermore, homogenized straw greatly reduced plant height (−36 %), leaf area (−49 %), SPAD (−49 %), and shoot weight (−53 %) than control at the late stage, but localized straw had little impact on plant performance. In conclusion, straw incorporation forms another soil enzyme activity hotspot (detritusphere) and promotes enzyme activities in the rhizosphere and soil profile. Collectively, homogenized straw incorporation intensified nutrient competition between microorganisms and roots and restricted plant performance.