Abstract
A theoretical consideration was undertaken to describe the applicability of a transient-state technique for establishing the maximum (virtual) growth rate in a continuously growing culture with special reference to inhibition kinetics. Transient states were applied by steadily increasing the dilution rate D by distinct steps, dD, within distinct time intervals, dt. As a result, it became evident that over a wide range of combination of both variables deviations from steady-state solutions ( μ max) were less than 10%. With an increase in the control strength of inhibition according to the Luong-model [1], even an approximation to the steady-state values was obtained. The results indicate that the transient-state method is useful in deriving kinetic parameters with appropriate accuracy in a rather short time as compared to steady-state treatment.
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