Abstract
Tomato yellow leaf curl virus (TYLCV) is a globally devastating disease that affects the production of many crops, particularly tomato, leading to considerable economic losses. RNA interference, which allows sequence-specific gene silencing at the post-transcriptional level, is an effective method to obtain virus-resistant lines of crops. Agrobacterium-mediated transient expression assay has emerged as a rapid and useful approach to evaluate gene functions in plants without the need to produce transgenic lines. In this study, we transformed the plant expression vector pBIN438-AV1–AC1–AC3(i/r), which consists of the inverted repeat of ΔAV1, ΔAC1, and ΔAC3 fusion fragments into Agrobacterium tumefaciens EHA105 to generate tomato lines with high viral resistance. The viral resistance of the fusion gene was evaluated by transient expression. The test plants did not show disease symptoms at 35 d postinoculation with TYLCV. This study provided an important method for plant antiviral breeding and safe tomato production.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
