Abstract
<p style="text-align: justify;">Leaf protoplasts, isolated from <em>Vitis vinifera</em> «Sakasly» and «Muscat d’Alexandrie» and protoplasts from embryogenic tissue of <em>Vitis</em> sp. «Seyval blanc» were incubated in the presence of PEG in a transformation solution containing the plasmid pBI426 which carries the β-glucuronidase (<em>gus</em>) and the neomycin phosphotransferase II (<em>npt</em>II) genes. The treated protoplasts were cultivated in CPW13 medium without kanamycin selection. 48h after the PEG transformation, transient expression of gus gene was detected histochemically and fluorimetrically in the protoplast cultures.</p>
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