Abstract
Expression of galactocerebroside (GC) and glial fibrillary acidic protein (GFAP) was studied in oligodendrocyte-enriched cultures of newborn mouse brains. In the cultures, GC was detectable as early as 1 day in vitro (1-DIV). Using double immunofluorescence labeling, some GC-positive cells were also stained homogeneously by the anti-GFAP serum. The intensity of GFAP staining increased until 9-DIV when the GC-positive cells revealed the typical morphology of oligodendrocytes, and the GFAP staining faded thereafter. The GFAP staining pattern of the GC-positive cells was not changed upon exposure to demecolcine, even though the GC-negative, GFAP-positive astrocytes showed perinuclear aggregation of GFAP. No intermediate filaments were observed in the oligodendrocytes by electron microscopy. The results suggest that the oligodendrocytes may have soluble GFAP in a certain period of early development.
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