Abstract
The leaf tissue of chrysanthemum (Dendrathema grandiflorum) cv. Tikara with the reporter gene b-glucoronidase (GUS) was transformed using 0.7 mm tungsten microprojectiles accelerated to high velocity with helium gas. Different pre-culture and bombardment conditions were evaluated. It was found that the appropriate pre-culture period was 3 days, the period in which the tissue acquires sufficient resistance to withstand physical damage inflicted by the microprojectiles. Using histochemical staining, transient expression of the GUS gene was detected in explants bombarded at a distance of 10 cm in a non-commercial biolistic system. The statistical tests indicated that some morphogenetic responses, as well as obtaining transformants with transient expression, depend on the distance and diameter of the microprojectile used during bombardment.
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