Abstract
A transient transfection system has been developed for a member of the Apicomplexa, Eimeria tenella, using β-galactosidase ( βgal) from Escherichia coli as the reporter enzyme. Successfully expressed constructs contained sequences of the E. tenella microneme gene Etmic-1 fused to the coding region of lacZ. Transfectants expressing βgal were able to invade host cells and proceed through part of the life-cycle, forming schizonts from which merozoites were released. This indicated that transfectants could differentiate at least to first generation schizonts. However, this differentiation was delayed compared with unelectroporated sporozoites by approximately 15 h. Some merozoites arising from transfected sporozoites also expressed βgal. These results are encouraging for the development of a stable transfection system for E. tenella, using βgal as a reporter enzyme.
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