Abstract
It is shown that the photoaccumulation of reduced pheophytin can occur in the isolated D1/D2/cytochrome b559 photosystem II (PSII) reaction centre when Fe(II) is used as a donor to P680 + in a way similar to that observed in the presence of sodium dithionite. Using Fe(II) as an electron donor system it has been possible to induce chlorophyll fluorescence transients from the isolated reaction centre complex when it is reconstituted with decylplastoquinone. These transients seem to reflect classical changes in fluorescence yield of PSII which accompany the conversion of the quinone acceptor from its oxidised to its reduced state. In the case of the isolated complex these decyplastoquinone dependent changes in fluorescence yield are dominated by direct quenching by oxidised quinone and do not reflect changes in the extent of the recombination reaction between the primary radical pair, P680 + pheophytin _.
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