Abstract
We discovered a transient adhesion property in poly(ethylene glycol) dimethacrylate (PEG-DMA) hydrogels and employed it to develop a novel “stem cell bandage” model of cellular delivery. First, we cultured human mesenchymal stromal cells (MSCs) on the surface of PEG-DMA hydrogels with high amounts of arginine-glycine-aspartic acid (RGD) adhesive peptides (RGD++) or without RGD (RGD-). On day 1, MSCs underwent an initial adhesion to RGD- hydrogels that was not significantly different over 13 days (n = 6). In addition, cells appeared to be well spread by day 3. Significantly fewer cells were present on RGD- hydrogels on day 15 compared to day 9, suggesting that RGD- hydrogels allow for an initial cellular adhesion that is stable for multiple days, but transient over longer periods with a decrease by day 15. This initial adhesion is especially surprising considering that PEG-DMA does not contain any biological adhesion motifs and is almost chemically identical to poly(ethylene glycol) diacrylate (PEG-DA), which has been shown to be non-adhesive without RGD. We hypothesized that MSCs could be cultured on RGD- PEG-DMA hydrogels and then applied to a wound site to deliver cells in a novel approach that we refer to as a “stem cell bandage”. RGD- donor hydrogels were successfully able to deliver MSCs to PEG-DMA acceptor hydrogels with high RGD content (RGD++) or low amounts of RGD (RGD+). Our novel “bandage” approach promoted cell delivery to these model surfaces while preventing cells from diffusing away. This stem cell delivery strategy may provide advantages over more common stem cell delivery approaches such as direct injections or encapsulation and thus may be valuable as an alternative tissue engineering approach.
Highlights
Numerous tissues have been targeted in tissue engineering approaches including cartilage [1], skin, bone [2], teeth [3], blood vessels [4], and intestine [5]
In the study of cellular attachment to poly (ethylene glycol) diacrylate (PEG-DA) hydrogels, we investigated the relative levels of attachment to RGD- PEG-DA hydrogels compared to RGD++ hydrogels
In the study of cellular attachment to PEG-DMA hydrogels, we examined the level of cellular attachment to RGD- PEG-DMA hydrogels and RGD++ PEG-DMA hydrogels
Summary
Numerous tissues have been targeted in tissue engineering approaches including cartilage [1], skin, bone [2], teeth [3], blood vessels [4], and intestine [5]. Mesenchymal stromal cells (MSCs) are commonly employed in tissue engineering. Transient adhesion on PEG-DMA for a stem cell bandage approach
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