Abstract
The present study investigated the chondroitin sulfate proteoglycan changes in the liver during acute liver injury induced by a one-shot administration of d-galactosamine in rats. Chondroitin sulfate proteoglycans were immunohistochemically stained with monoclonal antibodies, 1B5, 2B6, and 3B3, which recognize different stub structures of chondroitin sulfate chain exposed after treatment with chondroitinase ABC. The presence of biglycan, a dermatan sulfate/chondroitin sulfate proteoglycan, in the chondroitin sulfate proteoglycans was examined by determining the expression of biglycan gene by PCR and RNase protection assay. Immunoreactive chondroitin sulfate proteoglycans were detected in the ECM of portal areas and along the sinusoid, in either a radial or scattered dot pattern, out from the portal area. The positive signals in the portal area were detected in both control and injured rats. However, those along the sinusoid were detected only in injured rats, transiently from day 1 to 4 after the administration of d-galactosamine. The expression of biglycan gene was detected in both control and injured rats, and was four-five-fold the basal level at day 1 to 2 in the injured rats. These results suggest that the appearance of chondroitin sulfate proteoglycan along liver sinusoid is specific to acute liver injury, and biglycan is partially involved.
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