Abstract
Transgenic tomato plants (Solanum lycopersicum L. syn. Lycopersicon esculentum Mill. 'Yamal') expressed PR-5 protein genes (thau II, kiwi TLP) were obtained via Agrobacterium-mediated transformation. The presence of the foreign genes was detected by PCR; the accumulation of the PR-5 proteins in the leaves and fruits of transgenic plants was confirmed by western blot analyses. Seeds of T 1 and T 2 generation from transgenic plants with high expression of foreign proteins were obtained in the greenhouse. The biological effect of the foreign PR-5 proteins was verified by greenhouse testing where T 2 transgenic tomato plants demonstrated essentially enhanced disease resistance against Phytophthora infestans and Xanthomonas vesicatoria. Homozygous T 3 generation seeds from transgenic plants with high resistance were collected. As control of most diseases is extremely difficult, the feasibility of using PR-5 protein genes for genetic engineering plants with improved resistance to various phytopathogens will be discussed.
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