Abstract
Phytase is one of the most effective feed additives to increase the availability of phosphorus and minerals by catalyzing the hydrolysis of phytic acid. A modified appA gene (mappA) was transformed into soybean (Glycine max) under the control of a seed-specific promoter from common bean (Phaselous vulgaris). The soybean recombinant phytase showed optimal activity at pH 4.5 and 70 °C. A slight increase in enzyme activity occurred when the recombinant enzyme was pre-incubated with n-hexane. In addition, the phytase activity from our transgenic soybean does not reduce even after 2 hours of extraction with n-hexane at 55~65 °C. In conclusion, the oil extraction process using n-hexane does not inactivate the phytase expressed in the mAppA transgenic soybean, and the meal derived from the transgenic soybean processing can be used as feed supplement to livestock.
Highlights
Phytate is the major form of phosphorus found in plant-based feeds such as cereals, legumes and oilseed crops[1,2,3]
To create the transgenic soybean events expressing the mAppA, we constructed a T-DNA consisting of the phytase gene expression and the glyphosate resistance cassettes (Fig. 1)
Field studies showed that the addition of 500 to 1,000 units kg−1 of phytase to feed diet is analogous to 1% dicalcium phosphate or 0.18% phosphorus supplementation[5]
Summary
Phytate (myo-inositol 1, 2, 3, 4, 5, 6-hexakisphosphate) is the major form of phosphorus found in plant-based feeds such as cereals, legumes and oilseed crops[1,2,3]. Exogenous phytase is supplemented in the diet of monogastrics because of the absent or limited phytase activity in their digestive tract[12]. In this matter, phytase can improve the dietary availability of phosphorus and minerals and has emerged as one of the most effective feed additives[10,11]. Soybean meal is obtained after oil extraction as forage to livestock due its abundant protein content[21,22]. Soybean meal derived from mAppA transgenic soybean seeds can be used as sources of phytase as alternative to manual phytase addition after oil extraction
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