Abstract
Weeds burden plant growth as they compete for space, sunlight, and soil nutrients leading to 25–80% yield losses. Glyphosate [N-(phosphonomethyl)glycine] is a widely used broad spectrum non-selective herbicide that controls weeds by inhibiting 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) enzyme and interfering with the shikimate biosynthesis pathway. Cotton (Gossypium hirsutum L.) is one of the most important commercial crops grown worldwide for its fiber. We have developed herbicide tolerant transgenic cotton (cv. P8-6) by introgression of a codon-optimized and modified EPSPS gene (CP4-EPSPS) possessing an N-terminal chloroplast targeting peptide from Petunia hybrida. Because of the recalcitrant nature of cotton, a genotype-independent non-tissue culture-based apical meristem-targeted in planta transformation approach was used to develop transformants. Although in planta transformation methodologies are advantageous in developing a large number of transgenic plants, effective screening strategies are essential for initial identification of transformants. In the present study, the use of a two-level rigorous screening strategy identified 2.27% of T1 generation plants as tolerant to 800 and 1,500 mg/L of commercially available glyphosate (Roundup). Precise molecular characterization revealed stable integration, expression, and inheritance of CP4-EPSPS in advanced generations of the promising transgenic events. Further, superiority of selected transgenic plants in tolerating increasing levels of glyphosate (500–4,000 mg/L) was ascertained through reduced accumulation of shikimate. This report is the first of its kind where cotton transformants tolerating high levels of glyphosate (up to 4,000 mg/L) and accumulating low levels of shikimate have been identified. This study not only reiterated the genotype-independent nature of the transformation strategy but also reiterated the translational utility of the CP4-EPSPS gene in management of weeds.
Highlights
Cotton (Gossypium hirsutum L.) is one of the most important commercial crop plants grown worldwide for fiber and seeds
The present study demonstrates the utility of a modified CP4-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene in transgenic cotton and reiterates the applicability of the in planta transformation approach for cotton improvement
Apical meristem-targeted in planta transformation strategy was employed for the development of transgenic cotton
Summary
Cotton (Gossypium hirsutum L.) is one of the most important commercial crop plants grown worldwide for fiber and seeds. India is the second largest cotton producer contributing to approximately 22% of the world production (Directorate of Cotton Development Government of India, 2017; Mohapatra and Saha, 2019). Weeds are one of the very many factors that negatively challenge productivity of cotton accounting to approximately 50–85% of yield losses (Jain et al, 1981; Frisvold et al, 2009; Ayyadurai and Poonguzhalan, 2011; Awan et al, 2015; Ramachandra et al, 2016). Chemical herbicides are an effective way to control weeds, but have raised concerns of environmental pollution. To overcome these weeds, use of integrated weed management system in the field is an option (Sharma, 2008)
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