Abstract
Rice (Oryza sativa L.) cultivar Super Basmati was transformed with a cloned gene, Xa21, through particle bombardment method. Twenty-two plants were recovered from the selected calli. Initial screening through PCR confirmed the presence of the transgene in 21 plants. Southern analysis further confirmed the integration of the Xa21 gene into the genome of these transgenic plants. Analysis of the T1 generation revealed that both the hpt and Xa21 genes were successfully transmitted from the primary transformants to the progeny. Bioassays with two local isolates of bacterial leaf blight pathogen, Xanthomonas oryzae pv. oryzae (Xoo), showed that 14 transgenic lines were resistant to both Xoo isolates. No significant differences were observed in yield and grain characteristics between transgenic lines and the control; however, the transgenic lines took 8-12 days longer to flower and showed reduced plant height compared to the control plants. In conclusion, we have developed bacterial blight-resistant transgenic lines that can be further exploited by breeders for rice improvement.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
