Transgenerational effect of a tea saponin–matrine mixture promotes fecundity and alters associated bacteria in the fall armyworm Spodoptera frugiperda

Abstract

The fall armyworm Spodoptera frugiperda is a one of the most invasive and damaging pests that causes serious economic losses in several crops. Botanical insecticides such as tea saponin and matrine, as environmentally acceptable biopesticides, are widely used for controlling pests. Insecticide stress affects both individuals and the associated bacteria in insects. However, it remains unclear that if sublethal effects and bacteria are the drivers for the adaptive mechanisms of insecticides in fall armyworm. We examined the effectiveness of a 10:1 v/v mixture of biological insecticides against the fall armyworm and the impacts of several biological parameters and demographic variables of the armyworm targeting two generations at primary exposure. Moreover, the influence of the insecticide mixture on the associated bacteria of the fall armyworm was assessed via 16 S rRNA sequencing. The results showed that the mixture was highly toxic to fall armyworms, and the fecundity of the initially exposed generation (F0) significantly decreased; however, there was no significant effect on longevity. The larval duration, the total pre-reproductive period, longevity, fecundity, and mean generation time of the fall armyworm in the F1 significantly increased; yet the intrinsic and finite rates of the increase and the net reproductive rate remained fairly constant. Additionally, the population of associated bacteria was affected in both generations, with an increase in the microbial diversity within the fall armyworm compared to that of the F0 generation. Also, an increase in abundance of several genera of bacteria, including Acinetobacter, Serratia, Enterococcus, and Pseudomonas, was noted following treatment with the insecticide mixture. These results broaden our understanding of the impact of sublethal insecticide concentrations on the fall armyworm and its associated bacterial community.