Abstract
Transforming growth factor-beta-1 (TGF-beta-1) is a potent modulator of proliferation and differentiation in various tissues, and may be involved in the control of dental development and repair. This study was carried out to investigate the effects of TGF-beta-1 on alkaline phosphatase (ALPase) activity and mRNA level, and on DNA content in cultures of human pulp cells. Four lines of pulp cells (P1-P4), isolated from the upper wisdom teeth of four patients, were maintained separately in monolayer cultures in the presence of 10% fetal bovine serum. TGF-beta-1, at 0.1 ng/mL, increased ALPase activity and DNA content in P1 cultures, but not in P2-P4 cultures. In all cultures, TGF-beta-1, at 5 ng/mL, decreased ALPase activity to a very low level, and increased DNA content. Northern analysis showed that human pulp cells synthesized a single species of 2.6-kb liver/bone/kidney-type ALPase, and that TGF-beta-1, at 5 ng/mL, decreased the level of the ALPase mRNA. These results suggest that TGF-beta-1 is a mitogen for human pulp cells, and that it regulates the activity of the universal-type ALPase at the pre-translational level.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
