Abstract
The aim of this study was to investigate whether TGF-β1 regulates mouse GHRF secretion by primary cultures of placental cells from day 12 of pregnancy. Ten ng/ml TGF-β1 significantly inhibited mouse GHRF secretion by the third day of culture. The lowest concentration of TGF-β1 that significantly inhibited mouse GHRF secretion was 1 ng/ml. The inhibitory effect of TGF-β1 on mouse GHRF secretion was completely eliminated by addition of an anti-TGF-β1 antibody. Steady-state levels of mouse GHRF mRNA as assessed by Northern analysis was not reduced by incubation of placental cells from day 12 of pregnancy with 10 ng/ml TGF-β1 for 5 days. Both placenta and decidua expressed TGF-β1 mRNA, and the level of TGF-β1 mRNA in decidua increased during gestation while the level of TGF-β1 mRNA was constant throughout gestation. These findings suggest that TGF-β1 is one of the potent regulators of mouse GHRF secretion and that TGF-β1 regulates mouse GHRF secretion in an autocrine or paracrine manner in the mouse placenta in vivo.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
