Abstract
Abstract A Bacillus subtilis mutant (strain 168 try- thy-) with a thymine-adenine bias in complementary DNA strands was found. After BrU-incorporation into the DNA it was possible to separate physically hybrid DNA-molecules BrU-labeled in different complementary strands using CsCl density gradient centrifugation. These hybrid DNA preparations were used to investigate whether both strands can be informative during transformation. Taking advantage of the selective degradation of BrU-labeled DNA strands by long wavelength UV it was found that the loss of transforming acti vity was the same for both hybrid DNA preparations. Thus indicating that both complementary DNA strands can be genetically active during transformation. These results are supported by trans formation experiments with denatured and reannealed DNA-molecules and heteroduplex DNA-molecules.
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