Abstract
Amycolatopsis methanolicacontains a 13.29-kb plasmid (pMEA300) present both in the free state and integrated at a unique genomic location. A pMEA300-free derivative (strain WV1) was selected, allowing further analysis of pMEA300-encoded functions. Whole cells of strain WV1 could be transformed at high frequencies (approximately 106transformants per microgram of plasmid DNA) with both circular and linear plasmid DNA, provided that the pMEA300-encodedstf(stimulation of transformation frequency) gene was present.stfwould encode a putative protein of 373 amino acids withMr40,201, resembling putative regulatory proteins involved in sporulation ofStreptomyces griseusandStreptomyces coelicolor.
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