Abstract
An electric pulse was applied to transformation of yeast cell. Saccharomyces cerevisiae D13-1A ( a his3-532 trpl gal2) was used as a recipient strain for plasmid YRp7. Spheroplasts of S.cerevisiae D13-1A were placed under an electric pulse in the presence of YRp7. Transformants were observed when the pulse height was above 5 kV cm . The number of transformants increased with increasing pulse height and 945 transformants per ng DNA were obtained at 10 kV cm . Introduction of DNA might be caused by breakdown of the cell membrane under high electric voltage. Transformation Saccharomyces cerevisiae Spheroplast Plasmid Electric pulse
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