Abstract

The use of fossil fuels, as well as the environmental issues associated with their burning, has pushed for the development of clean, renewable energy sources. Biofuels made from lignocellulosic biomass are considered a carbon-neutral and sustainable method. As the demand for non-petroleum fuels grows, more attention will be placed on developing a cost-competitive liquid transportation biofuel like ethanol. This study was conducted to produce bioethanol utilizing the SHF (separate hydrolysis and fermentation) technique from corn stove lignocellulose. Pretreatment with sodium hydroxide at various concentrations was also studied. The influence of enzymatic saccharification, fermentation time, and substrate concentration on sugar yield and, eventually, ethanol production was investigated. Fermentation was carried out by using the enzymatically saccharified hydrolysate and monoculture of Saccharomyces cerevisiae. The results reveal that pretreatment with 2% NaOH followed by 48 hours of hydrolysis produced the maximum bioethanol production (30.21 ±0.13 g/L). This study findings indicated that alkali-pretreated corn stove might be used as a feedstock for bioethanol production, reducing reliance on fossil fuels.

Highlights

  • Increasing interest in biomass fuel has grown in recent decades as the price of petroleum-derived gasoline has risen

  • Lignocellulosic biomass appears to be an appealing alternative for inexhaustible biofuel supplies, reducing reliance on fossil fuel resources (Ramaraj et al, 2015; Manmai et al, 2019)

  • Lignocellulose is a chemical compound found in plants that are made up of polysaccharides that are tightly linked by lignin

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Summary

Introduction

Increasing interest in biomass fuel has grown in recent decades as the price of petroleum-derived gasoline has risen. Second-generation biofuels come from nonfood crops (Manmai et al, 2020a, b). The phrase 2nd generation is used loosely in the scientific literature to refer to feedstocks, conversion pathways, and products (Mejica et al, 2021; Trejo et al, 2021). Lignocellulosic biomass appears to be an appealing alternative for inexhaustible biofuel supplies, reducing reliance on fossil fuel resources (Ramaraj et al, 2015; Manmai et al, 2019). The lignocellulosic biomass feedstock is abundant, recyclable, inexpensive, and spread uniformly throughout nature (Nong et al, 2020; Saengsawang et al, 2020). The raw material for biorefinery products is lignocellulosic material (Dussadee et al, 2014; 2016; 2017; Bhuyar et al, 2021)

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