TRANSFORMATION OF IAAM GENE INTO ANTHER-DERIVED EMBRYOS OF LOQUAT MEDIATED BY AGROBACTERIUM TUMEFACIENS

Abstract

The parthenocarpic gene iaaM was transferred into anther-derived embryos, using Agrobacterium-mediated transformation. The results showed that 2-3-day pre-culture, 15-min infection and 3-day co-culture with 10 mg L-1 AS in the co-culture medium led to an increase in transformation efficiency. Cefotaxime at 250 mg L-1 or Ampicillin at 750 mg L-1 were optimal. Sixteen transgenic lines were obtained. The iaaM gene-specific amplification results showed that 9 strains were amplified at the expected target bands, and the PCR positive rate was 56.3%. The PCR-Southern analysis indicated that 8 strains showed hybridization signal, which further proved that the iaaM gene was integrated into the genome of the embryos, and the transformation rate was about 0.5%.