Transformation of green monkey kidney cells by SV40 genome: The establishment of transformed cell lines and the replication of human adenoviruses and SV40 in transformed cells

Abstract

Green monkey kidney cells were transformed by ultraviolet-inactivated adeno 7-SV40 hybrid virus and SV40. Transformed cells were obtained either as piled-up foci in monolayers of normal cells or as surviving colonies among degenerated cells. From these foci or colonies, many transformed cell lines were established. These transformed cells contained SV40 T antigen but not SV40 virion antigen. Approximately 40–50% of SV40 genome was transcribed in 3 transformed cell lines. SV40 could not be detected or rescued from the transformed cells by contact-culture or cell-fusion with green monkey kidney cells, except for one clone which gave variable results. All the transformed cell lines supported the efficient replication of human adenovirus types 2, 7, and 12, while nontransformed cells did not. Transformed cell lines established from foci supported the efficient replication of SV40. However, transformed cell lines established from surviving colonies were resistant to superinfection with SV40 or SV40 DNA.