Transformation of eIF5B1 gene into Chrysanthemum to gain calluses of high temperature tolerance

Abstract

Chrysanthemum is one of the most important ornamental flowers in the world. In general, abnormal morphology of Chrysanthemum flowers is developed during~35–40 °C, which significantly damages incomes of flower gardener. Therefore, to select Chrysanthemum lines of high temperature tolerance is of vital importance. In this study, transgenic calluses with eIF5B1, a regulatory gene of high temperature tolerance, were generated, and the presence of the transgene and its stable expression were confirmed through PCR, GUS assay and Western blotting. These transgenic calluses with plantlets were also observed by both electron and optical microscope. Findings indicate these lines contain more starch granules, which are a marker of high temperature tolerance. We expect that the efficient protocol developed in this study will facilitate the genetic transformation of the genes of interest and that these regulatory genes will help in keeping the flower morphology of Chrysanthemum under high temperature stress.