Transformation of diploid human fibroblasts by transfection with the v- sis, PDGF2/c- sis, or T24 H- ras genes

Abstract

Gene transfection techniques have provided powerful methods to examine the roles of cellular and retroviral oncogenes in the transformation process in rodent fibroblasts. However, the use of such techniques with diploid human fibroblasts has been limited. We have developed transfection procedures to reproducibly transfect such cells with oncogenes, and methods for the biological characterization of the transformants. We have shown that the v- sis and T24 H- ras oncogenes, as well as the platelet-derived growth factor gene ( PDGF2/c- sis, are capable of inducing a transformed phenotype in normal diploid human fibroblasts, but are not capable of conferring infinite lifespan or making such cells tumorigenic.