Abstract

Digitaria sanguinalis, an African grass, was micropropagated from immature inflorescences and callus was transformed by particle gun bombardment. Integration and expression of the bar and uidA genes was shown in the T0 generation. Transformants were shown to be fertile by the production of viable seeds. The time taken from bombardment to the setting of seed was approximately four months. D. sanguinalis and its transformants were shown to be sensitive to maize streak virus (MSV). Therefore this is an ideal model system for testing genetically engineered resistance to MSV.

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