TRANSFORMATION OF DEHYDROEPIANDROSTERONE INTO DIHYDROTESTOSTERONE BY ISOLATED CELLS FROM RAT PREPUTIAL GLAND

Abstract

After the rat preputial gland was treated with collagenase and trypsin, five bands of cells were isolated by centrifugation in Ficoll gradients. Homogenates of the heavier cells (Bands IV and V) which contained less lipids, were more active than the homogenates of the lighter cells (Bands I, II and III) in transforming [1,2-3H]-dehydroepiandrosterone ([1,2-3H]-DHA) into [3H]-androstenedione and [3H]-testosterone and the latter into [3H]-dihydrotestosterone (DHT). In the presence of NAD, NADH and NADPH-generating system, [1,2-3H]-DHA was transformed into [3H]-DHT in 50–60% yield by homogenates of cells in Bands IV and V. DHT levels in the preputial gland were measured by radioimmunoassay. The levels in female rats reduced by 77% from 3.14 ± 0.27 to 0.72 ± 0.10 pg/mg tissue after adrenalectomy, and by 45% to 1.71 ± 0.10 pg/mg tissue after ovariectomy. In male rats, the level reduced by 15% from 4.58 ± 0.55 to 3.88 ± 0.62pg/mg tissue after adrenalectomy and by 40% to 2.74 ± 0.21 pg/mg tissue after orchidectomy. These results demonstrated the transformation of DHA into DHT in the preputial gland of the rat, and that the adrenal is an important source of precursor steroid (DHA) for DHT formation in the preputial gland.