Abstract
Solgi T., Moradyar M., Zamani M.R., Motallebi M. (2015): Transformation of canola by chit33 gene towards improving resistance to Sclerotinia sclerotiorum. Plant Protect. Sci., 51: 6–12. An endochitinase gene ( chit 33-cDNA) from the biocontrol fungus Trichoderma atroviride was overexpressed un der the CaMV35S constitutive promoter in canola (R line Hyola 308). Transformation of cotyledonary petioles was achieved via Agrobacterium tumefaciens . The insertion of the transgene was verified by PCR and Southern blotting. The transgenic over-expression approach was used in order to investigate antifungal activity of expressed Chit33 on Sclerotinia sclerotiorum . Antifungal activity was detected in transgenic canola using detached leaf assay. Lesion sizes of transgenic canola caused by S. sclerotiorum were significantly retarded when compared to non-transgenic canola plant.
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