Transformation of aqueous methyl orange to green metabolites using bacterial strains isolated from textile industry effluent

Abstract

The present report is based on the degradation of methyl orange, which is categorized as an acute toxic compound and mutagenic substance. Since it is highly solubilized in the environment, it is very challenging to remove. This study is focusedon biological treatment which has the ability to breakdown methyl orange. Three bacterial strains were initially isolated from the textile wastewater. Out of those strains, Aeromonas hydrophila showed maximum degradation of about 85%. The optimized conditions for the degradation process were found to be 20 ppm concentration, pH 7, and temperature 35 °C. The carbon source such as 1% sucrose is found to be appropriate carbon source for the degradation. While nitrogen source suited well was found to be 1% peptone over 1% meat extract. The metabolite obtained after degradation was analyzed using FT-IR and GC–MS. N, N-dimethyl p-phenylenediamine and 4-amino sulfonic acid were identified as the end products.