Abstract
The antisense wheat Mlo gene, Ta-Mlo, was transformed into wheat (Tritivum aestivum L.) varieties Yangmai 158 and Jimai 20 via biolistic transformation using immature embryo calli as explants. After two rounds of bialaphos selection and regeneration, herbicide-resistant plants were obtained, which were subsequently confirmed by PCR, PCR-Southern hybridization, genomic dot hybridization, and BASTA resistance analysis. The results showed that the Ta-Mlo antisense transgenic Yangmai 158 and Jimai 20 plants were obtained. The real time fluorescence quantitative PCR analysis proved that the transcript of Ta-Mlo was knocked down in these transgenic plants. The disease resistance test showed that the six transgenic lines appeared highly resistance to powdery mildew pathogen Blumeria graminis f. sp. tritici (Bgt). The transgenic lines showed distinct acceleration of the production and stabilization of papillae, and effective suppression to further development of haustoria of Bgt. Therefore, the transgenic lines showed high resistance to Bgt.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
