Abstract
DNA was extracted from Bacillus stearothermophilus, strain BS-1, a derivative of ATCC 12980, by shaking cells with a mixture of chloroform and iso-amylalcohol in the presence of sodium deoxycholate and partially purified by the treatment with RNase A. This DNA preparation was shown to induce genetic transformation of the amylase-deficient mutants which were made competent by culturing cells in a semi-defined medium devised by the author specifically for this bacterial species.
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