Abstract
The key role of cell cultures in different scientific fields is worldwide recognized, both as in vitro research models alternative to laboratory animals and substrates for biological production. However, many safety concerns rise from the use of animal/human cell lines that may be tumorigenic, leading to potential adverse contaminations in cell-derived biologicals.In order to evaluate the suitability of 13 different cell lines for Poliovirus vaccine production, safety and quality, in vitro/in vivo tumorigenicity and Poliovirus propagation properties were evaluated.Our results revealed that non-human primate cell lines CYNOM-K1, FRhK-4, 4MBr-5 and 4647 are free of tumorigenic features and represent highly susceptible substrates for attenuated Sabin Poliovirus strains. In particular, FRhK-4 and 4647 cell lines are characterized by a higher in vitro replication, resulting indicated for the use in large-scale production field.
Highlights
Poliomyelitis is a highly contagious disease caused by a virus of the Enterovirus genus, belonging to the Picornaviridae family, known as Poliovirus and composed by a 7,500 nucleotides (+) single stranded RNA molecule [1,2]
Immortalization of animal and human cells, derived from primary cell cultures, is a phenomenon mainly due to genetic mutations or infections by oncogenic viruses, which can result in the appearance of transformed features and tumorigenic properties
Cell cultures are widely used as substrates for the production of many biotechnological and biological products for human use, such as viral vaccines
Summary
Poliomyelitis is a highly contagious disease caused by a virus of the Enterovirus genus, belonging to the Picornaviridae family, known as Poliovirus and composed by a 7,500 nucleotides (+) single stranded RNA molecule [1,2]. No specific therapy is available against the virus, but effective inactivated and attenuated vaccines are essential to prevent the disease. Immortalization of animal and human cells, derived from primary cell cultures, is a phenomenon mainly due to genetic mutations or infections by oncogenic viruses, which can result in the appearance of transformed features and tumorigenic properties. Cells can undergo several modifications during in vitro cultivation, resulting in the appearance of novel
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