Transformation and mineralization of synthetic 14C-labeled humic model compounds by soil-feeding termites

Abstract

The majority of termite species are considered to be humivorous, but the exact nature of their carbon and energy source, the mechanisms involved in digestion and the impact of this feeding habit on the humification of soil organic matter are largely obscure. We performed feeding trials with soil-feeding termites (Termitidae: Termitinae), using 14C-labeled humic model compounds as substrates. In the case of Cubitermes orthognathus, the components of chemically identical synthetic humic acids (HA), labeled either in their proteinaceous or aromatic building blocks, and synthetic fulvic acids (FA), labeled in the aromatic building blocks, all had similarly low mineralization rates (2.5, 2.2 and 2.7%, respectively) when incubated in soil for 10 days in the absence of termites. When termites were present, the mineralization rate of the aromatic component of HA and FA increased only slightly but significantly (2.4 and 3.1%, respectively; P < 0.05), whereas that of the proteinaceous component increased more than 10-fold (30.8%). Similar results were obtained when the protein was peptonized prior to polymerization (9.7 vs. 27.4% mineralization). Mineralization of HA was accompanied by a transformation of the residual peptide label to FA, whereas the aromatic label of HA and FA was partly transformed to humin during gut passage. High-performance gel permeation chromatography showed a strong shift in the size-class distribution of peptide label towards low-molecular-weight products, especially of the material ingested by the termites; the smallest molecules were recovered from the termite bodies. Similar results were obtained in feeding trials with Cubitermes umbratus and Thoracotermes macrothorax. Together with previous findings, the current results provide strong evidence that during gut passage, the combined action of extreme alkalinity in the anterior hindgut, autoxidative processes, and probably also proteolytic activities, renders a large reservoir of potential substrates accessible. While peptidic components of humic substances are selectively digested, aromatic components are apparently not an important food source for soil-feeding termites. These findings have important implications for the mobilization of organic nitrogen in tropical soils.