Abstract
Delivery of vaccine is a major impediment to ensuring vaccine efficacy and compliance. Existing vaccine delivery approaches have their limitations and these led to the development of novel approaches for vaccine delivery. Non-invasive vaccine delivery is in demand and for this skin appears to be a potential sites that elicit immune responses. Topical application of antigen and adjuvant directly on the skin is termed as topical vaccination. It has potential to reduce physical skin penetration by injection and provide potentially effective vaccines. In the present study novel carrier, transfersomes were used for topical delivery of tetanus toxoid (TT). Transfersomes were prepared by reverse phase evaporation method and they were evaluated for shape, size, entrapment efficiency and deformability index. Transfersomal formulation with optimal concentration of Soya phosphatidylcholine (SPC) and sodium deoxycholate (85:15 w/w) showed entrapment efficiency of 39.8±0.032 and deformability index of 16.4. In-vivo study revealed that topically given TT containing transfersomes after boosting produced immune responses (0.877±0.081) that is as good as that produced by subcutaneously given alum adsorbed TT (0.948±0.088). Furthermore, fluorescence microscopy has confirmed the bioactive delivery through the skin layers. A brief comparative study has been done with liposomes.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
