Abstract
In glasshouse studies we have previously shown that endosperm-specific RNAi suppression of the primary starch phosphorylation enzyme, Glucan, Water Dikinase (GWD) leads to enhanced early vigor, greater leaf biomass, and increases in both head size and yield. To confirm these affects in a field setting, trials were conducted in three Australian environments. Field results were consistent with those in the glasshouse for increased flag leaf area and rachis nodes. However, there was also a decrease in tiller number and consequently a decrease in yield for one event at two sites. These findings provide potentially important information on plant vigor enhancement and highlight the challenges of transferring the modification of complex traits from single plants in controlled environments to the field.
Highlights
Wheat has been an important component of the human diet for thousands of years
Wheat has been found in human settlement pits over 8000 years old [1]
We have developed transgenic wheat lines with endosperm-specific downregulation of a primary enzyme involved in starch degradation, Glucan, Water Dikinases (GWD) [12]
Summary
Wheat has been an important component of the human diet for thousands of years. Wheat has been found in human settlement pits over 8000 years old [1]. We have developed transgenic wheat lines with endosperm-specific downregulation of a primary enzyme involved in starch degradation, Glucan, Water Dikinases (GWD) [12]. 7, 82developed transgenic wheat lines with endosperm-specific downregulation 2ofof a7 primary enzyme involved in starch degradation, Glucan, Water Dikinases (GWD) [12]. Excess (SEX4), LikeSexFour and 2 (LSF1 and 2)), modify the level of phosphate groups in [13,14,15,16], allowing access to degradative enzymes including Endo (α) and Exo (β) amylases [16]. We observed that in glasshouse studies inactivation of GWD in endosperm of wheat led to increases in early vigor, plant biomass and yield, associated with the endosperm of wheat led to increases in early vigor, plant biomass and yield, associated with alteration of head architecture including head size in a single-pot glasshouse trial. Environments, and discuss the importance of measuring transgenic traits in real-world settings
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