Abstract
Introduction. Pulasan and lychee are from the same family and closely related, therefore the SSR markers are expected to be highly transferable between these two taxa. We investigated the transferability of 12 lychee (Litchi chinensis Sonn.) simple sequence repeat (SSR) loci to pulasan (Nephelium ramboutan-ake L.). Materials and methods. Genomic DNA was extracted from 20 accessions of pulasan for the PCR amplification of the SSR loci using 12 pairs of SSR primers derived from lychee. The PCR products were resolved on dena- turing polyacrylamide gels. Results. The percentage of SSR transferability from lychee to pulasan was 58.3% and the percentage of polymorphic SSR markers was 25%. Discussion. The moderate transferability and low polymorphism rates suggest the possibility of interrup- tions within the repeat motif and mutations in the flanking sequences of SSR repeat motifs. Our results did not reveal a high rate of transferability between lychee and pulasan. However, this study showed that the SSR markers developed in lychee are a good source of molecular markers for pulasan. Malaysia / Litchi chinensis / Nephelium ramboutan-ake / molecular biology / genetic markers / PCR / genetic polymorphism
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