Abstract
Emmer is the tetraploid wheat (genome constitution: AABB) from which hexaploid or common wheat (Triticum aestivum L., genome:AABBDD) was derived. We examined transferability of 180 microsatellite markers from hexaploid wheat to wild emmer wheat [T. turgidum ssp. dicoccoides (Korn. Ex Asch. & Graebner) Thell.] and cultivated emmer wheat [T. turgidum ssp. dicoccum (Schrank ex Shobler) Thell.]. Among the 180 primer pairs, 121 (67.2%) amplified microsatellite loci from the ssp. dicoccoides genome and 122 (67.8%) from the ssp. dicoccum genome. Between these two subspecies, polymorphic banding patterns were observed at 97 (53.5%) microsatellite loci. Five primer pairs (2.8%) produced amplification products only from the ssp. dicoccoides genome while 7 other primer pairs (3.9%) produced products only from the ssp. dicoccum genome. Fifty microsatellite markers (27.8%) failed to amplify any products in these two subspecies of T. turgidum. The microsatellite markers we identified to show cross-species transferability should be useful in studies of gene mapping and genome evolution in tetraploid wheat.
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