Transferability of Brassica-Derived Microsatellites to Related Genera and their Implications for Phylogenetic Analysis

Abstract

Sixty-six sequence tagged microsatellite sites (STMS) from three different Brassica species (Brassica napus L., Brassica nigra (L.) Koch and Brassica rapa L.) were evaluated for transferability to Lepidium sativum L., Brassica tournefortii Gouan., Sinapis alba L., B. nigra and Eruca sativa (Mill.) Thell. representing four different genera of the family Brassicaceae. In total, 56 (84.8%) STMS markers amplified satisfactorily, of which 32 (48.5%) showed transferability to at least one of the five different species considered for the study. The cross-transferable STMS markers were applied to evaluate the phylogenetic relationships between them. The results indicated a high level of congruence with the earlier reports with regard to genetic divergence and groupings of taxa, revealing that even small number of STMS markers is enough to adequately establish the systematic positions of different genera/species of the family Brassicaceae. Cross-transferability of sixty-six sequence tagged microsatellite sites (STMS) derived from Brassica napus L., Brassica nigra (L.) Koch and Brassica rapa L. was evaluated using five different species of Brassica and allied genera. A total of 32 (48.5%) STMS markers showed cross-transferability to at least one of the five different species considered for the study. Phylogram constructed using cross-transferable markers revealed a high level of resemblance with the earlier reports, establishing the significance of these markers in phylogenetic analysis of the family Brassicaceae. Cross-transferabilty of B. napus derived STMS marker NA14-F11 and NA14-G02. Lane M–100-bp DNA ladder, Lane 1–Lepidium sativum, Lane 2–Brassica tournefortii, Lane 3–Salix alba, Lane 4–B. nigra, Lane 5–Eruca sativa.