Abstract
Byrsonima Rich. is one of the largest genera of the Malpighiaceae family with 97 species occurrence in Brazil and multiple potentialities, including pharmaceutical and food industries. In this study, 17 microsatellite markers characterized in Byrsonima cydoniifolia were tested for seven related taxa, all species are native to Brazil and four are endemic. Genomic DNA was extracted from leaves tissues and 17 microsatellite markers were used to cross-amplification of microsatellite regions. Polymorphism and genetic diversity were evaluated for B. intermedia, B. verbascifolia, B. laxiflora, B. subterranea, B. umbellata, B. linearifolia. from 16 individuals and for B. viminifolia from 14 individuals. Transferred microsatellite markers panels ranged from 11 (64.8%) in B. viminifolia to 6 (35.2%) in B. umbellata. The total number of alleles per locus ranged from 5 (B. linearifolia) to 8 (B. subterranea) alleles. B. umbellata showed lower values of observed and expected heterozygosity (HO = 0.312; HE = 0.436) and B. subterranea presented the highest values (HO = 0.687; HE = 0.778). A greater number of microsatellite markers should be developed for B. umbellata. The microsatellite marker panels transferred to the species B. intermedia, B. verbascifolia, B. laxiflora, B. subterranea, B. viminifolia and B. linearifolia are very informative, with a high combined probability of exclusion of paternity (Q ≥ 0.976) and the low combined probability of identity (I ≤ 9.91 × 10-6), potentially suitable for future genetic-population studies, supporting strategies for maintaining the genetic diversity and for exploration of Byrsonima species as genetic resources.
Highlights
Byrsonima Rich., one of the largest genus belonging to the Malpighiaceae family, contains approximately 150 species, distributed from Mexico to South America
Aiming at the importance of developing future strategies of conservation and economic exploitation of the numerous species of the genus Byrsonima sp., in the present study we reported the ability of SSR markers developed for Byrsonima cydoniifolia [7] (Bernardes et al, 2014) to amplify SSR loci in other seven Byrsonima species
The presence of non-specific amplification was found for almost all species, with the exception of B. umbellata and B. viminifolia, ranging from three loci (17.7%) in B. intermedia to one locus (5.8%) in B. subterranea and B. linearifolia (Table 1)
Summary
Byrsonima Rich., one of the largest genus belonging to the Malpighiaceae family, contains approximately 150 species, distributed from Mexico to South America. SSR markers are widely used for detecting genetic diversity and population structure, gene flow patterns, the incidence of genetic drift, paternity analysis, linkage of phenotypic and genotypic variation, identification of conservation units, and creation of germplasm banks [5] (Semagn et al, 2006) These markers are potentially transferable among related taxa, due to the homologous nature of the DNA sequence in the flanking regions of the microsatellites. We characterized the genetic variability of the microsatellite regions transferred for each species, in order to determine how informative are the transferred loci These polymorphic markers can be used to conduct effective conservation strategy, domestication planning, parentage studies, and creation of germplasm bank using Byrsonima sp. These polymorphic markers can be used to conduct effective conservation strategy, domestication planning, parentage studies, and creation of germplasm bank using Byrsonima sp. as a genetic resource, and to unveil phylogenetic problems for its related taxa
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