Transfer of Teichococcus ludipueritiae and Muricoccus roseus to the genus Roseomonas, as Roseomonas ludipueritiae comb. nov. and Roseomonas rosea comb. nov., respectively, and emended description of the genus Roseomonas

Abstract

Phylogenetic analyses based on 16S rRNA gene sequences revealed that Teichococcus ludipueritiae and Muricoccus roseus are closely related to the species of the genus Roseomonas. The type strain of Teichococcus ludipueritiae, 170/96(T), exhibited 16S rRNA gene sequence similarity levels of 96.4 % to Roseomonas cervicalis ATCC 49957(T), 95.0 % to Roseomonas aquatica TR53(T), 94.5 % to Muricoccus roseus 173/96(T), 93.4 % to Roseomonas mucosa ATCC BAA-692(T) and 93.5 % to Roseomonas gilardii subsp. gilardii ATCC 49956(T), while Muricoccus roseus 173/96(T) showed 16S rRNA gene sequence similarity values of 95.7 % to R. mucosa ATCC BAA-692(T), 95.7 % to R. aquatica TR53(T) and 95.3 % to R. gilardii subsp. gilardii ATCC 49956(T) and R. gilardii subsp. rosea ATCC BAA-691(T). Different phylogenetic analysis methods (neighbour-joining, maximum-likelihood and maximum-parsimony) confirmed that both species are within the Roseomonas branch. Neither polyamine patterns (spermidine predominant) nor major characteristics in the polar lipid profiles distinguished the two species from representatives of the genus Roseomonas. The fatty acid composition of the two species exhibited alphaproteobacterial characteristics but, like Roseomonas species, they also showed considerable amounts of the rarely encountered C(18 : 1) 2-OH. On the other hand, they showed some phenotypic differences, but their features are compatible with the transfer of these two species to the genus Roseomonas. We propose the reclassification of Teichococcus ludipueritiae and Muricoccus roseus as Roseomonas ludipueritiae comb. nov. (type strain 170/96(T) =CIP 107418(T) =DSM 14915(T)) and Roseomonas rosea comb. nov. (type strain 173/96(T) =CIP 107419(T) =DSM 14916(T)), respectively. Emended descriptions of the genus Roseomonas and the species Roseomonas gilardii (and its subspecies Roseomonas gilardii subsp. gilardii and Roseomonas gilardii subsp. rosea), Roseomonas aquatica, Roseomonas cervicalis, Roseomonas mucosa and Roseomonas lacus are provided.