Transfer of nif genes from Nitrogen fixer Azotobacter chroococcum to phosphors solubilizing Bacillus megaterium var. phosphaticum by conjugation

Abstract

The probability of transferring the nitrogen fixation genes (nif genes) from Azotobacter chroococcum to Bacillus megaterium Var phosphaticum were explored In this research. A total of 50 isolated of Azotobacter sp. from many soil samples of Erbil city-Iraq, were subjected to cultural, morphological and biochemical characterization. The nitrogen fixing genes (nifH1, nifH2, nifU, nifV and FV genes) which located in chromosomal DNA from Azotobacter chroococcum were transferred through the conjugation process to the Bacillus megaterium Var phosphaticum as recipient cell . The presences of (nif genes) was confirmed by gene detection tests to the donor and recipient genomes and transconjugants cell by PCR amplification of nifH1, nifH2, nifU, nifV and FV genes . The transconjugant cells were showed the ability and the effectively action on nitrogen fixation when they growth on solid N2 free medium, and solubilizing phosphorus.