Transfer of four isolates of Bursaphelenchus doui into Monochamus alternatus and potential vector switching of the nematode

Abstract

Summary To reinforce vector-switching potential of Bursaphelenchus doui in its ecological and evolutionary contexts, we tested our previous hypothesis that “B. doui (or its ancestor) was transferred by Acalolepta fraudatrix, Acalolepta sejuncta, and/or Monochamus subfasciatus (or their ancestral species) from broad-leaved trees to conifers, switched vectors from these cerambycid beetles to Monochamus beetles in conifers, and then evolved into the common ancestor of Bursaphelenchus mucronatus and Bursaphelenchus xylophilus”. The affinity of four B. doui isolates and one B. xylophilus isolate for Monochamus alternatus was tested using our simple nematode-loading method to the beetle, and the affinity was assessed based on the nematode loads on the beetles. Phoretic stages of two B. doui isolates obtained from Monochamus saltuarius and Pinus densiflora showed loading levels similar to that of B. xylophilus, which were significantly higher than those of the other two B. doui isolates obtained from A. fraudatrix and M. subfasciatus. This result indicates that the first two isolates of B. doui derived from a conifer-using beetle and a coniferous tree adapt to M. alternatus better than the last two isolates associated with beetles using broad-leaved trees. The data reinforce that vector switching of B. doui could have occurred during the evolutionary history of the B. xylophilus group.