Abstract
DNA isolated from Choleraphage ø149 of Group IV was infectious when mixed with competent V. cholerae cells. The cells were competent during mid-log phase of growth. The infectivity of phage DNA was destroyed by deoxyribonuclease but not by ribonuclease or pronase. About 5 min is required for the establishment of the DNase resistant state. The dose response curve for transfection suggested that 2 to 3 molecules of DNA are required to produce one infections center. An infectivity of 5 × 10 4 infectious center per μg of DNA was obtained.
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