Abstract
The development of a transfection system for the protozoan parasite Plasmodium falciparum hailed a breakthrough in understanding one of the world's most prevalent human pathogens. One hundred years after the discovery by Sir Ronald Ross that the etiological agent of malaria is transmitted to humans by the bite of infected Anopheline mosquitoes, we now have the tools at hand with which to investigate the biology and pathogenicity of P. falciparum, at the molecular level, in an attempt to develop sustainable means to control this important infectious disease. Here we review the recent progress facilitated by transfection in malariology and discuss the future opportunities this technique offers.
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