Transfection of FHTT into human MCF-7 cell line and its biological characteristics

Abstract

Objective To construct FHIT eukaryotic expression vector and transfect FHIR gene into human MCF-7 breast carcinoma cell line.and analyze the effects of FHIT on MCF-7.Methods FHIT eukaryotic expression vector pcDNA3.1(+)/FHIT Was constructed.FHIT full length cDNA was transfected into MCF-7 cell line by the vector of pcDNA3.1(+)/FHIT,and the transfectant with stable expression of FHIT was obtained by clone selection.The effects of FHIT on breast carcinoma cells were analyzed.including the alteration of MCF-7 cell lines in cell cycle.apoptosis and rate of growth.Results pcDNA3.1 (+)/FHIT was successfully constructed.and the expression of FHIT in MCF-7 cells inhibited the growth of breast carcinoma cells.Compared with MCF-7 cells.MCF-7/FHIT cells had significandy decreased number in S and G2/M-phases.and increased number in G0/G1-phase.The apoptosis rate of MCF-7/FHIT and MCF-7 Was(29.75±5.90)%,and(11.21±6.10)% resepctively.As compared with MCF-7 cells.the apoptotic MCF-7/FHIT cells were significantly increased.Conclusion Transfection of FHIT inhibited growth and proliferation,and induce apoptosis of breast carcinoma cells MCF-7. Key words: Frangile histidine triad; Eukaryotic expressive vector; Gene transfection; Breast carcinoma