Transfected adenosine A 1 receptor-mediated modulation of thrombin-stimulated phospholipase C and phospholipase A 2 activity in CHO cells

Abstract

Thrombin receptor activation in Chinese hamster ovary (CHO) cells stimulates the hydrolysis of inositol phospholipids and the release of arachidonic acid. Our previous studies have shown that activation of the human transfected adenosine A 1 receptor in CHO cells (CHO-A1) potentiates the accumulation of inositol phosphates elicited by endogenous P 2U purinoceptors and CCK A receptors. In this study we have investigated whether adenosine A 1 receptor activation can modulate thrombin-stimulated arachidonic acid release and/or inositol phospholipid hydrolysis in CHO-A1 cells. Thrombin stimulated [ 3H]arachidonic acid release and total [ 3H]inositol phosphate accumulation in CHO-A1 cells. Both these responses to thrombin were insensitive to pertussis toxin. The protein kinase C activator, phorbol 12-myristate 13-acetate (PMA), potentiated thrombin-stimulated [ 3H]arachidonic acid. In marked contrast, PMA inhibited thrombin-stimulated [ 3H]inositol phosphate accumulation. The selective protein kinase C inhibitor Ro 31-8220 (3-{1-[3-(2-isothioureido)propyl]indol-3-yl}-4-(1-methylindol-3-yl)-3-pyrrolin-2,5-dione) had no effect on thrombin-stimulated [ 3H]arachidonic acid release but reversed the potentiation of thrombin-stimulated [ 3H]arachidonic acid release elicited by PMA. The selective adenosine A 1 receptor agonist N 6-cyclopentyladenosine (CPA) augmented the release of [ 3H]arachidonic acid produced by thrombin. Co-activation of the adenosine A 1 receptor also potentiated thrombin-stimulated [ 3H]inositol phosphate accumulation. The synergistic interactions between the adenosine A 1 receptor and thrombin were abolished in pertussis-toxin-treated cells. The potentiation of [ 3H]arachidonic acid release by CPA was blocked by the protein kinase C inhibitors Ro 31-8220 and GF 109203X (3-[1-[3-(dimethylamino)propyl]-1 H-indol-3-yl]-4-(1 H-indol-3-yl)-1 H-pyrrole-2,5-dione). In conclusion, thrombin receptor activation in CHO-A1 cells stimulates the accumulation of [ 3H]inositol phosphates and the release of [ 3H]arachidonic acid through pertussis-toxin-insensitive G-proteins. Experiments using PMA suggest that protein kinase C differentially regulates thrombin receptor activation of phospholipase C and phospholipase A 2. Co-activation of the transfected human adenosine A 1 receptor augments thrombin-stimulated phospholipase C and phospholipase A 2 activity. Finally, the augmentation of phospholipase A 2 activity by the adenosine A 1 receptor is inhibited by selective protein kinase C inhibitors, suggesting the involvement of protein kinase C.