Abstract
The expression level of human granurocytes macrophage colony stimulating factor (hGM-CSF) mRNA in Chinese hamster ovary (CHO) DR1000L4N cells increased by 24% with pressurization. Treatment of cells with chelerythrine chloride (10 nM, 15 min), an inhibitor of protein kinase C (PKC), did not suppress the pressure-induced (0.9 MPa) expression of hGM-CSF mRNA, while it decreased the hGM-CSF gene expression level at normal pressure (0.1 MPa). Treatment with U0126 (20 μM,, 60 min), a specific inhibitor of extracellular signal-related kinase (ERK1/2), decreased the expression level of hGM-CSF mRNA at 0.1 MPa to 80% of that without U0126 treatment. Similarly, treatment with U0126 decreased the pressure-induced (0.9 MPa) expression level of hGM-CSF mRNA to 79% of the control expression level at 0.1 MPa without treatment. The amount of intracellular phosphorylated ERK1/2 increased with pressurization (0.9 MPa). These results suggest that the pressure-induced expression of hGM-CSF mRNA in CHO DR1000L4N cells depends not on PKC but on the ERK1/2 signaling cascade.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
