Transduction inDrosophilaolfactory receptor neurons is invariant to air speed

Abstract

In the vertebrate nose, increasing air speed tends to increase the magnitude of odor-evoked activity in olfactory receptor neurons (ORNs), given constant odor concentration and duration. It is often assumed that the same is true of insect olfactory organs, but this has not been directly tested. In this study, we examined the effect of air speed on ORN responses in Drosophila melanogaster. We constructed an odor delivery device that allowed us to independently vary concentration and air speed, and we used a fast photoionization detector to precisely measure the actual odor concentration at the antenna while simultaneously recording spikes from ORNs in vivo. Our results demonstrate that Drosophila ORN odor responses are invariant to air speed, as long as odor concentration is kept constant. This finding was true across a >100-fold range of air speeds. Because odor hydrophobicity has been proposed to affect the air speed dependence of olfactory transduction, we tested a >1,000-fold range of hydrophobicity values and found that ORN responses are invariant to air speed across this full range. These results have implications for the mechanisms of odor delivery to Drosophila ORNs. Our findings are also significant because flies have a limited ability to control air flow across their antennae, unlike terrestrial vertebrates, which can control air flow within their nasal cavity. Thus, for the fly, invariance to air speed may be adaptive because it confers robustness to changing wind conditions.